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il 1racp protein isoforms  (Cullinan Oncology)
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Il 1racp Protein Isoforms, supplied by Cullinan Oncology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 1racp d5  (Santa Cruz Biotechnology)
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Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein
Il 1racp D5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 1racp d5 - by Bioz Stars, 2026-09
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mouse-anti il-1racp (d5) sc-376872  (Santa Cruz Biotechnology)
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Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein
Mouse Anti Il 1racp (D5) Sc 376872, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse-anti il-1racp (d5) antibody sc-376872  (Santa Cruz Biotechnology)
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Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein
Mouse Anti Il 1racp (D5) Antibody Sc 376872, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard antibody (rabbit mab against il-1racp, anti il12rbeta1 antibody: il-12rbeta1 antibody; cat. no. gtx103917)  (GeneTex)
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Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein
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Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein
Mouse Monoclonal Anti Fbxo4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein

Journal: Nature communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling.

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein

Article Snippet: Antibodies used for pulldown assay include rabbit anti-fibromodulin antibody (H50) (sc-33772, Santa Cruz Biotechnology, Santa Cruz, CA; 1:500 dilution), goat antihuman IL-1 beta/IL-1F2 antibody (AF-201-NA, R&D systems; 0.1 μg/mL), rabbit anti-IL1 receptor I/IL-1R-1 antibody (ab154524; Abcam; 1:1000 dilution), mouse-anti IL-1RAcP (D5) (sc-376872, Santa Cruz Biotechnology; 1:100 dilution), donkey anti-goat IgG (H + L) secondary antibody, HRP (A15999, Thermo Fisher Scientific; 1:5000 dilution), donkey anti-rabbit IgG (H+ L) secondary antibody, HRP (A16023, Thermo Fisher Scientific; 1:5000 dilution), and donkey anti-mouse IgG (H + L) cross-adsorbed secondary antibody, HRP (SA1-100, Thermo Fisher Scientific; 1: 2000 dilution).

Techniques: Pull Down Assay, Incubation, In Vitro, Recombinant, Membrane

Fig. 10 | Fibromodulin (FMOD) promotes the direct binding of interleukin 1 receptor type 1 (IL1R1) and interleukin-1 receptor accessory protein (IL1RAP) on the surface of BJ-myofibroblasts. All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1RAP-proximity liga- tion assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b–g Quantification of IL1R1:IL1RAP-PLA+ signal per cell at 1- (b), 6- (c), 12- (d), 24- (e), 48- (f), and 72-h (g)

Journal: Nature communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling.

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: Fig. 10 | Fibromodulin (FMOD) promotes the direct binding of interleukin 1 receptor type 1 (IL1R1) and interleukin-1 receptor accessory protein (IL1RAP) on the surface of BJ-myofibroblasts. All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1RAP-proximity liga- tion assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b–g Quantification of IL1R1:IL1RAP-PLA+ signal per cell at 1- (b), 6- (c), 12- (d), 24- (e), 48- (f), and 72-h (g)

Article Snippet: Antibodies used for pulldown assay include rabbit anti-fibromodulin antibody (H50) (sc-33772, Santa Cruz Biotechnology, Santa Cruz, CA; 1:500 dilution), goat antihuman IL-1 beta/IL-1F2 antibody (AF-201-NA, R&D systems; 0.1 μg/mL), rabbit anti-IL1 receptor I/IL-1R-1 antibody (ab154524; Abcam; 1:1000 dilution), mouse-anti IL-1RAcP (D5) (sc-376872, Santa Cruz Biotechnology; 1:100 dilution), donkey anti-goat IgG (H + L) secondary antibody, HRP (A15999, Thermo Fisher Scientific; 1:5000 dilution), donkey anti-rabbit IgG (H+ L) secondary antibody, HRP (A16023, Thermo Fisher Scientific; 1:5000 dilution), and donkey anti-mouse IgG (H + L) cross-adsorbed secondary antibody, HRP (SA1-100, Thermo Fisher Scientific; 1: 2000 dilution).

Techniques: Binding Assay, In Situ, Staining